The mobivision mkindex command can be used to construct reference genomes, and specifying different values for the -m parameter will result in different sizes of the constructed reference genome. The larger the -m value, the larger and faster the reference genome will be. The default -m value is 16. With default parameters, the reference genome folder size for the human genome is approximately 19GB. The command to build the reference is:

mobivision mkindex -n GRCh38 -f GRCh38.primary_assembly.genome.fa -g gencode.v38.primary_assembly.annotation.gtf -r human-gencode-v1.0

It is recommended to use FASTA and GTF files from Gencode or Ensembl for reference genome construction. The GTF annotation file should include at least exon, gene, and transcript information.