MobiVision v3.2 introduces the new integrate command, expanding the command set. It includes a new cutadapt sequence trimming step and improves the filtering methods for cleaner polyA removal. BAM file content is adjusted with additional unmapped reads information and new tags, improving mapping information and sequencing saturation calculations. The H5AD file is updated to include full matrix information. The cell_metrics file and total genes detected information are added and written to the summary.csv file. HTML report species information and mapping information parameters are adjusted to be closer to CellRanger's settings. The alignment rate is higher, and the analysis results are better.